Regeneration of Phaseolus vulgaris from epicotyls and hypocotyls via direct organogenesis

Bamboo Regeneration via Embryogenesis and Organogenesis

Callus Induction and Plant Regeneration of Chrysanthemum morifolium and C. coccineum via Direct and Indirect Organogenesis and Genetic Fidelity Analysis Using IRAP, ISSR and SCoT Molecular Markers

In vitro propagation of C. morifolium cv. ‘Homa’ and cv. ‘Delkash’ and wild C. coccineum via direct and indirect organogenesis and somatic embryogenesis were investigated. BAP at 0, 1, 2 and 3 mg l-1 or NAA at 0, 0.05, 0.1 and 0.2 mg l-1 concentrations were used to induce direct and indirect organogenesis of shoot tip explants. To study the callu...

Glycosidases of Phaseolus vulgaris

The enzymes, cr-galactosidase, P-galactosidase, a-mannosidase, P-glucosidase, and p-acetylglucosaminidase, have been purified from the germinating seeds of Phaseolus vulgaris. All five enzymes have been simultaneously isolated in a highly active form. The pH optimum, K,,,, and energy of activation of each glycosidase for the reaction of hydrolysis of the appropriate P-nitrophenyl glycoside have...

Rescue of early heart-shaped embryos and plant regeneration of Phaseolus polyanthus Greenm. and Phaseolus vulgaris L

In order to improve plant recovery from early heart-shaped Phaseolus embryos, several refinements were made to the embryo rescue technique developed previously in our laboratory. Using P. polyanthus Greenm. and P. vulgaris L. genotypes, a 20% increase in the germination rate was obtained by using Phillips salts and 25 μg.l-1 ABA. This germination medium improved the development of the embryos a...

Uromyces phaseoli and Phaseolus vulgaris

To study the function of bean rust haustoria either the host, Phaseolus vulgaris, or the parasite, Uromyces phaseoli, were labeled with 3H-lysine. 24h and 48 h after inoculation samples of the infected leaves were taken and processed for electronmicroscopic autoradiography. When the host was labeled, silver grain densities over the structures of host and parasite were compared. Very low grain d...